Occurrence of denitrifying polyphosphate-accumulating bacteria in wastewater treatment plants in the Czech Republic
DOI:
https://doi.org/10.35933/ENTECHO.2022.003Abstract
Key technology for maintaining surface water quality is nitrogen and phosphorus removal from wastewater. The most common and simplest technology is biological nitrogen removal and chemical precipitation of phosphorus. In this work, occurrence of bacteria Candidatus Accumulibacter phosphatis, which can remove both of these nutrients, was monitored. The occurrence of these organisms and use of their potential in the biological nutrient removal can bring significant benefits to the operation of wastewater treatment plants, such as reduced dose of coagulating agents, lower sludge production and lower demands on aeration of the oxic part of the technology. This work is focused on monitoring of the occurrence of these organisms in wastewater treatment plants in the Czech Republic. Fluorescence in-situ hybridization was used to monitor and evaluate these bacteria, because conventional methods are not sufficient. The implementation of this method is described in the experimental part and obtained results are further discussed.
References
Daims, H.; Lücker, S.; Wagner, M., 2006. Daime, a novel image analysis program for microbial ecology and biofilm research. Environmental Microbiology 8(2), 200–213. https://doi.org/10.1111/j.1462-2920.2005.00880.x
Dockx, L.; Caluwé, M.; Dobbeleers, T.; Dries, J., 2022. Nitrous oxide formation during simultaneous phosphorus and nitrogen removal in aerobic granular sludge treating different carbon substrates. Bioresource Technology 345, 126542. https://doi.org/10.1016/j.biortech.2021.126542
Flowers, J. J.; He, S.; Yilmaz, S.; Noguera, D. R.; McMahon, K. D., 2009. Denitrification capabilities of two biological phosphorus removal sludges dominated by different ‘Candidatus Accumulibacter’ clades. Environmental Microbiology Reports 1(6), 583–588. https://doi.org/10.1111/j.1758-2229.2009.00090.x
Kim, J. M.; Lee, H. J.; Kim, S. Y.; Song, J. J.; Park, W.; Jeon, C. O., 2010. Analysis of the Fine-Scale Population Structure of “Candidatus Accumulibacter phosphatis” in Enhanced Biological Phosphorus Removal Sludge, Using Fluorescence In Situ Hybridization and Flow Cytometric Sorting. Applied and Environmental Microbiology 76(12), 3825–3835. https://doi.org/10.1128/AEM.00260-10
Kim, J. M.; Lee, H. J.; Lee, D. S.; Jeon, C. O., 2013. Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal. Applied and Environmental Microbiology 79(6), 1969–1979. https://doi.org/10.1128/AEM.03464-12
Lanham, A. B.; Moita, R.; Lemos, P. C.; Reis, M. A. M., 2011. Long-term operation of a reactor enriched in Accumulibacter clade I DPAOs: performance with nitrate, nitrite and oxygen. Water Science and Technology 63(2), 352–359. https://doi.org/10.2166/wst.2011.063
Murnleitner, E.; Kuba, T.; van Loosdrecht, M. C. M.; Heijnen, J. J., 1997. An integrated metabolic model for the aerobic and denitrifying biological phosphorus removal. Biotechnology and Bioengineering 54(5), 434–450. https://doi.org/10.1002/(SICI)1097-0290(19970605) 54:5<434::AID-BIT4>3.0.CO;2-F
Rubio-Rincón, F. J.; Lopez-Vazquez, C. M.; Welles, L.; van Loosdrecht, M. C. M.; Brdjanovic, D., 2017. Cooperation between Candidatus Competibacter and Candidatus Accumulibacter clade I, in denitrification and phosphate removal processes. Water Research 120, 156–164. https://doi.org/10.1016/j.watres.2017.05.001
Seviour, R. J.; Mino, T.; Onuki, M., 2003. The microbiology of biological phosphorus removal in activated sludge systems. FEMS Microbiology Reviews 27(1), 99–127. https://doi.org/10.1016/S0168-6445(03)00021-4
van Loosdrecht, M. C. M.; Nielsen, P. H.; Lopez-Vazquez, C. M.; Brdjanovic, D. (Ed.), 2016. Experimental methods in wastewater treatment. IWA Publishing, London.
Welles, L.; Lopez-Vazquez, C. M.; Hooijmans, C. M.; van Loosdrecht, M. C. M.; Brdjanovic, D., 2016. Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions. AMB Express 6(1), 44. https://doi.org/10.1186/s13568-016-0214-z
Yun, G.; Lee, H.; Hong, Y.; Kim, S.; Daigger, G. T.; Yun, Z., 2019. The difference of morphological characteristics and population structure in PAO and DPAOgranular sludges. Journal of Environmental Sciences 76, 388–402. https://doi.org/10.1016/j.jes.2018.06.003
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Dominik Matýsek, Miroslava Česká, Iveta Růžičková
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
